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ORIGINAL ARTICLE
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J Korean Dent Assoc. 2023;61(4):276-284. Published online March 31, 2023.
DOI: https://doi.org/10.22974/jkda.2023.61.4.002
- 치아 및 치주복합체 항상성에서 phosphatase and tension homolog (PTEN)의 역할
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김예현
, 최원준
, Ni Xiong
, 임원희
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서울대학교 치의학전문대학원 치과교정학교실, 서울대학교 치과병원 교정과
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- Effect of phosphatase and tension homolog (PTEN) on periodontal complex
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Ye-hyun Kim
, Wonjun Choi
, Ni Xiong
, Won Hee Lim
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Department of Orthodontics, School of Dentistry, Seoul National University, Department of Orthodontics, Seoul National University Dental Hospita
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- Corresponding author: Won Hee Lim Tel: +82 2 2072 0272, Fax: +82 2 2072 3817, Email: whlim@snu.ac.kr
- Received: January 26, 2023; Revised: January 31, 2023 Accepted: March 3, 2023.
- Abstract
- Purpose
Phosphatase and tension homolog (PTEN) has been known to play a role of maintenance of homeostasis over the body. It is little known about the roles of PTEN in periodontal complex. The purpose of this study was to explore the role of PTEN in periodontal complexes after removing PTEN from osteoblasts and odontoblasts.
Materials and Methods
The resulting OCN‐Cretg/+;Ptenfl/fl mice were created after by conditionally eliminating the PTEN gene using an Osteocalcin (OCN) Cre driver and the resulting OCN‐Cretg/+;Ptenfl/fl mice (experimental group) were compared with the wild type mice (control group) using micro-CT, histology and immunohistochemical analyses. The amount of dentin and alveolar bone was analyzed, and osteopontin and bone sialoprotein in the periodontal ligament space (PDL) were evaluated and collagen in the PDL was analyzed quantitatively and qualitatively.
Results
Micro CT showed that dentin and bone were significantly accumulated in an experimental group. Dentin sialo-protein was significantly increase in dentin of an experimental group. Internal balance in the PDL was altered with increased expression of PCNA in an experimental group.
Conclusions
Loss of PTEN causes an increase of dentin and bone in the periodontal complexes with an alteration of internal balance in the PDL. These findings underscore the effect of PTEN on homeostatic control of periodontal complexes.
Keywords :PTEN; homeostasis; periodontal complex
